منابع مشابه
Automated Assay of Glycosidasesl
Automated methodology for monitoring the presence of one or more glycosidases in biological extracts has been described (l-7). These procedures require use of large amounts of synthetic glycoside(s) (l-4,6,7), compound automated systems (5), or extensive mechanical and/or electrical alterations of existing apparatus (8). The present paper describes a simple modification of the Technicon Basic A...
متن کاملGlycosidases of Aspergillus niger
A highly purified preparation of 1,2-oc-L-fucosidase, free of /3-galactosidase and P-N-acetylglucosaminidase activities, has been obtained from a commercial preparation of Aspergillus niger by a simple isolation procedure involving ammonium sulfate precipitation, pressure dialysis, repeated gel Cltration on Sephadex G-150, and chromatography on diethylaminoethyl Sephadex A-50. The enzyme has a ...
متن کاملGlycosidases of Phaseolus vulgaris
The enzymes, cr-galactosidase, P-galactosidase, a-mannosidase, P-glucosidase, and p-acetylglucosaminidase, have been purified from the germinating seeds of Phaseolus vulgaris. All five enzymes have been simultaneously isolated in a highly active form. The pH optimum, K,,,, and energy of activation of each glycosidase for the reaction of hydrolysis of the appropriate P-nitrophenyl glycoside have...
متن کاملGlycosidases – A Mechanistic Overview
Carbohydrates are the most abundant and structurally diverse class of biological compounds in nature. However, our current understanding regarding the relationship between carbohydrate structure and its biological function is still far from what is known regarding proteins and nucleic acids. Initially, carbohydrates were only recognized as structural and energy storage molecules (e.g. cellulose...
متن کاملAutomated assay of hexosaminidases in serum.
In serum there are two classes of hexosaminidase. One of these is heat-labile and is inactive in children with Tay-Sachs disease. Serum from heterozygotes for Tay-Sachs disease has half the normal activity of the heat-labile enzyme. The heat-labile enzyme can be inactivated by heating serum diluted in buffer for 5 mm at 60 #{176}C. We have used this property to develop a reliable, fully automat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1978
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90852-7